Medical Biofilm Techniques 2016, ESCMID Postgraduate Technical Workshop

22 - 25 August 2016, Lyngby, Denmark

  • ESCMID Study Group for Biofilms (ESGB)
Course Coordinators
  • Thomas Bjarnsholt, Copenhagen, Denmark
  • Claus Sternberg, Lyngby, Denmark
Course Objectives

The course focuses on medical biofilms with examples from urinary tract infections, osteomyelitis, chronic wound and cystic fibrosis lung infections. The techniques include set-up and running of biofilm flow cell systems, fluorescence in situ hybridization, various advanced imaging techniques/analysis, and demonstrations of biofilm techniques in experimental animal research. There will be morning and late afternoon lectures. The rest of the day will be devoted to laboratory demonstrations and hands-on work.

Course Venue

Technical University of Denmark
building 301, 1st floor, room 056
2800 Lyngby, Denmark

Target Audience

Maximum 20 scientists (including clinical and basic research) with experience in laboratory training.

CME Accreditation

The organizers of the course will apply for European CME accreditation through EACCME.

Contact Person (Scientific Programme)

Prof Thomas Bjarnsholt
Department for Clinical Microbiology
Juliane Maries Vej 22
2100 Copenhagen

Phone +45 206 598 88
Fax +45 354 564 12

Administrative Secretariat

Kasper Nørskov Kragh
University of Copenhagen
Department of International Health, Immunology and Microbiology
Blegdamsvej 3b
2200 Kopenhagen, Denmark

Phone +45 228 441 51

Course Programme

Presentations are availabe in the ESCMID Online Lecture Library.

Monday, 22 August 2016

08:30 Coffee
09:00 Welcome & introduction to medical biofilms: antibiotic resistance and medical importance in general. Thomas Bjarnsholt
10:00 Exercises, practical and technical information: biofilms and tools, confocal microscopy (Imaris image processing). Janus Haagensen, Kasper Kragh & Claus Sternberg
12:00 Lunch
13:00 Tour in the institute and practicals in the lab:

  • Building biofilm set-ups (5 teams will build one system each)
  • Medium preparation for biofilm systems

15:00 Inoculation of already prepared and sterilized system 1 (E. coli and mutants):

  • Microtiter assay: inoculation of 2 plates, one that you will proceed with fixation and one without (E. coli and mutants, each team makes their own plates)
  • Analysis of alginate bead biofilms

18:00 End of day 1

Tuesday, 23 August 2016

09:00 Cystic fibrosis and biofilms: clinical and experimental scenarios. Susanne Häussler & Helle Krogh Johansen
11:00 Confocal microscopy and image acquisition of the E. coli strains (biofilm system 1, images for COMSTAT. Microscopy continues throughout the rest of the day).
Janus Haagensen & Claus Sternberg
12:00 Lunch
13:00 Sterilization of biofilm systems:

  • Addition of antibiotics to biofilm system 2 (inoculated 3 days ago)
  • Microtiter assay: washing and reading (OD measurement and crystal violet staining)
  • Analysis of alginate bead biofilms

17:00 Chronic osteomyelitis and biofilms. Mark Shirtliff
17:30 Novel anti-biofilm strategies infections. Tom Coenye
18:00 End of day 2

Wednesday, 24 August 2016

09:00 Medical biofilms: genetic and physiology. Helle Krogh Johansen
10:00 - How to do FISH of biofilms. Kasper Kragh
          - Demo of COMSTAT. Claus Sternberg
11:00 - Images for COMSTAT (biofilm system 1) using confocal microscopy
            (one team at the time)
          - Treating microtiter data
12:00 Lunch
13:00 - Imaging of antibiotic treated biofilm (system 2)
          - COMSTAT of obtained data from system 1, Imaris on images from system 1 and 2
18:00 End of day 3
18:30 Dinner

Thursday, 25 August 2016

09:00 Hydrodynamics of biofilms. Paul Stoodley
10:00 Animal models of biofilm infections. Claus Moser, Thomas Bjarnsholt and Mark Shirtliff

  • Pseudomonas aeruginosa biofilm infections
  • Lung infection models
  • Peritoneal implant model
  • Wound infection model

12:00 Lunch
13:00 Evaluation of experiments

  • Mortality/euthanasia, bacteriology, pathology, Xenograph model, lung function testing
  • Inflammation/immunology
  • Image processing/demonstration of biofilms from animal models

17:00 End of course and certificate of participation

Biofilm system 1

E. coli, E. coli F+ traD mutant, image acquisition and COMSTAT (15 channel system, Media FB with Fe-EDTA + A-10. Addition of glucose, proline & thiamine). System assembled and sterilized.

Biofilm system 2

P. aeruginosa (Gfp) alone for antibiotic treatment and P. aeruginosa (Yfp) + P. aeruginosa pilA (Cfp) for structure development, image acquisition after antibiotic treatment (15 channel system, Media FB with trace metals + A-10. Addition of glucose.). System assembled, sterilized and inoculated.

Faculty Members
  • Thomas Bjarnsholt, Copenhagen, Denmark
  • Tom Coenye, Ghent, Belgium
  • Janus Haagensen, Copenhagen, Denmark
  • Susanne Häussler, Hannover, Germany
  • Kasper Kragh, Copenhagen, Denmark
  • Helle Krogh Johansen, Copenhagen, Denmark
  • Claus Moser, Copenhagen, Denmark
  • Mark Shirtliff, Baltimore, MD, United States
  • Claus Sternberg, Copenhagen, Denmark
  • Paul Stoodley, Ohio, OH, United States