26 - 30 August 2013, Villars-sur-Ollon, Switzerland

Course Objectives

• Review the current knowledge on the epidemiology and clinical presentation of infections due to intracellular bacteria with special focus on Coxiella, Bartonella, Ehrlichia, Rickettsia, Chlamydia and Tropheryma whipplei.
• Know the limitations and advantages of each diagnostic approach that may be used to document these bacterial infections.
• Know how to manage and treat patients infected with one of these strict intracellular bacteria.
• Understand the pathogenesis of infections due to these intracellular bacteria.
• Appreciate the importance of genomics in understanding the biology and virulence of these intracellular bacteria.
• Discuss cases in interactive meet-the-expert sessions.
• Have practicals introducing key important tools used to establish the diagnosis of infections due to intracellular bacteria as well as to discover new bacterial species.
  

Course Venue

EUROTEL VICTORIA 4****
Route des Layeux
1884 Villars-sur-Ollon, Switzerland

Phone +41 (0)24 495 31 31
Fax +41 (0)24 495 39 53
Eurotel
Website

On Friday morning for the practicals: University Hospital of Lausanne
Lausanne, Switzerland

Target Audience

30–50 clinical microbiologists and infectious diseases specialists interested in intracellular bacteria as well as researchers interested in this field.

Prof Gilbert Greub
Médecin-chef, MD-PhD
Institut de Microbiologie
de l’Université de Lausanne
Centre hospitalier universitaire vaudois (CHUV)
Bugnon 48
1011 Lausanne, Switzerland

Phone +41 (0)21 314 49 79
Mobile +41 (0)79 556 17 95
Fax +41 (0)21 314 40 60
Gilbert Greub

Administrative Secretariat

Martine Moreillon
Dept. of Fundamental Microbiology
Bâtiment Biophore – UNIL-Sorge
1015 Lausanne, Switzerland

Phone +41 (0)21 692 56 00
Mobile +41 (0)79 515 07 19
Fax +41 (0)21 692 56 05
Martine Moreillon

Presentations are availabe in the ESCMID Online Lecture Library.

Monday, 26 August 2013

Epidemiology

• 14:00 Introduction. A. Letaief & G. Greub
• 14:10 Rickettsia around the world. P.-E. Fournier
• 14:50 Transmission of chlamydiae by arthropods. A. Croxatto
• 15:10 Treatment and control of chlamydial and rickettsial infections in sheep and goats. D. Longbottom
• 15:30 Break
• 16:00 Lessons from the outbreak in the Netherlands on transmission & epidemiology of Q fever. M. Nabuurs
• 16:40 The Val de Bagnes Coxiella outbreak. O. Peter
• 17:00 The Coxiella outbreak in Lavaux. G. Greub
• 17:20 Break
• 17:45 4 clinical quizzes. A. Letaief, O. Peter, M. Nabuurs & F. Fenollar

Tuesday, 27 August 2013

Clinical presentation, diagnostic approaches and treatment (Part 1)

• 08:30 Miscarriage & intracellular bacteria. D. Baud
• 09:10 Listeria infections. M. Lecuit
• 09:50 Break
• 10:20 Negative-blood culture endocarditis. P.-E. Fournier
• 11:00 Chronic Q fever. C. Bleeker
• 11:40 Whipple disease. F. Fenollar
• 12:00 Bartonella infections. G. Greub
• 12:20 Lunch

Pathogenesis & biology

• 13:50 Listeria pathogenesis. M. Lecuit
• 14:30 Secretion systems of intracellular bacteria. A. Croxatto
• 14:50 Ehrlichia pathogenesis. J. S. Dumler
• 15:10 Pathogenesis of rickettsial infections. G. Greub
• 15:30 Break
• 16:00 Pathogenesis of Bartonella infections. P.-E. Fournier
• 16:20 Pathogenesis of Chlamydia pneumoniae. M. Puolakkainen
• 16:40 Catalases and intracellular bacteria. B. Rusconi
• 17:00 Importance of Pmps in Chlamydia pathogenesis. D. Longbottom
• 17:20 Tropheryma whipplei pathogenesis. F. Fenollar
• 17:40 Break
• 18:00 3 clinical quizzes. P.-E. Fournier, M. Lecuit & C. Bleeker

Wednesday, 28 August 2013

Clinical presentation, diagnostic approaches and treatment (Part 2)

• 08:30 Ehrlichia infections. J. S. Dumler
• 09:10 Rickettsial spotted fever. A. Letaief
• 09:50 Murine and endemic typhus. P.-E. Fournier
• 10:10 Break
• 10:40 Legionella and other amoebae-resisting microorganisms. G. Greub
• 11:20 Acute Q fever. M. Nabuurs
• 11:40 Serological diagnosis of Q fever. O. Peter
• 12:00 Lunch
• 14:00 Excursion to the Swiss mountains (20 min train and 1–2 h walk)
• 18:00 3 clinical quizzes. G. Greub, M. Puolakkainen & D. Longbottom

Thursday, 29 August 2013

Clinical presentation, diagnostic approaches and treatment (Part 3)

• 08:30 Chlamydia pneumoniae. M. Puolakkainen
• 09:10 Chlamydia trachomatis. D. Baud
• 09:50 Break
• 10:20 Chlamydia abortus: from ovines to humans. D. Longbottom
• 10:40 Waddlia chondrophila: pathogenic role, diagnosis & treatment.
  D. Baud
• 11:00 Parachlamydia: pathogenic role, diagnosis & treatment. G. Greub
• 11:20 Rhabdochlamydia and Simkania: a role in pneumonia?
  M. Puollakkainen
• 11:40 Lunch

Genomics

• 13:30 How to sequence a genome? G. Greub
• 13:50 Functional genomics of intracellular bacteria. M. De Barsy
• 14:10 Genomics of Rickettsia. P.-E. Fournier
• 14:30 Genomics of Ehrlichia. J. S. Dumler
• 14:50 Genomics of Legionella. C. Bertelli
• 15:10 Genomics of Whipple. F. Fenollar
• 15:30 Break
• 16:00 Genomics of Listeria. M. Lecuit
• 16:20 Genomics of Chlamydia. D. Longbottom
• 16:40 Genomics of Chlamydia-related bacteria. C. Bertelli
• 17:20 Genomics of Bartonella. P.-E. Fournier
• 17:40 Break
• 18:00 3 clinical quizzes. D. Baud, J. S. Dumler & G. Greub
• 19:30 Gala Dinner

Friday, 30 August 2013

Practicals in Lausanne
Each practical will take 20 minutes – these are rather practical demos than practical work. All participants (max 50) will be divided into 9 groups, i.e. 5 to 6 persons per group.

• 08:45 Transfer from Villars to Lausanne (<1h)
• 09:50 Practicals 1 to 3
• 10:50 Break to change location
• 11:00 Practicals 4 to 6
• 12:00 Lunch
• 13:00 Practicals 7 to 9
• 14:00 Farewell & coffee

At the institute of Microbiology

• P1: Amoebal co-culture. S. Aeby & L. Pilloux
• P2: Amoebal enrichment: migration front. N. Jacquier
• P3: Cell culture: epithelial cells incl. trypsination. B. Rusconi

At the Bacteriology ward

• P4: MALDI-TOF identification of strict intracellular bacteria: the Chlamydiales example. G. Prod’hom
• P5: Bioinformatics for dummies: how to assemble a genome and how to annotate a genome. C. Bertelli & T. Pillonel
• P6: Legionella, Listeria, ...: phenotypes and phenotypic identification in the diagnostic laboratory. O. Opota

At the School of Medicine

• P7: Dissection of ticks. O. Peter
• P8: Doing a Gram & Gimenez staining. M. De Barsy & C. Kebbi
• P9: Immunofluorescence. A. Croxatto

• David Baud, Lausanne, Switzerland
• Claire Bertelli, Lausanne, Switzerland
• Chantal Bleeker, Nijmegen, The Netherlands
• Antony Croxatto, Lausanne, Switzerland
• John Stephen Dumler, Baltimore, United States
• Florence Fenollar, Marseille, France
• Pierre-Edouard Fournier, Marseille, France
• Gilbert Greub, Lausanne, Switzerland
• Marc Lecuit, Paris, France
• Amel Letaief, Sousse, Tunisia
• David Longbottom, Edinburgh, United Kingdom
• Marrigje Nabuurs, Nijmegen, The Netherlands
• Olivier Peter, Sion, Switzerland
• Mirja Puolakkainen, Helsinki, Finland