Clostridium Difficile: Practical Aspects of Diagnostics, Typing and Comparative Genomics, ESCMID Postgraduate Technical Workshop

2 - 4 September 2015, Maribor, Slovenia

  • Austrian Agency for Health and Food Safety (AGES), Vienna, Austria
  • Faculty of Medicine, University of Maribor, Slovenia
  • National Laboratory for Health, Environment and Food (NLZOH), Maribor, Slovenia
  • ESCMID Study Group for Clostridium difficile (ESGCD)
Course Coordinators
  • Franz Allerberger, Vienna, Austria
  • Maja Rupnik, Maribor, Slovenia
Course Objectives

Provide young microbiologists with an updated overview of typing methods for Clostridium difficile. In a practical hands-on approach the participants will be trained in in-vitro susceptibility testing, agarose-based ribotyping, sequencer-based capillary ribotyping, molecular toxin gene detection, and use
of comparative genomics for epidemiological investigations in the daily infection control practice.

Course Venue

Faculty of Medicine
University of Maribor
Taborska 8
2000 Maribor

Target Audience

15 – 20 young clinical microbiologists, infectious diseases specialists and technicians interested in Clostridium difficile, who want to get an updated
overview of the theoretical and practical use of diagnostic approaches, molecular typing methods and comparative genomics for Clostridium difficile.

Contact Person (Scientific Programme)

Prof Dr Maja Rupnik
Department of Microbiology
Faculty of Medicine, University of Maribor
Taborska Ulica 8
2000 Maribor

Phone +38 624 500 183
Fax + 38 624 500 193

Administrative Secretariat

Sandra Janezic PhD
National Laboratory for Health,
Environment and Food (NLZOH)
Prvomajska 1
2000 Maribor

Phone +38 624 602 346
Fax +38 624 500 193

Course Programme


Presentations are availabe in the ESCMID Online Lecture Library.


Wednesday, 2 September 2015

12:00 Registration (lunch provided in registration area)
13:00 Welcome and introduction. Franz Allerberger & Maja Rupnik
13:15 Clostridium difficile infection – underestimated public health problem.
           Franz Allerberger
13:35 ECDIS-net: European surveillance of Clostridium difficile infections.
           Ed Kuijper
14:00 Basic diagnostics. Frédéric Barbut
14:45 Break
15:00 Different platforms for agarose-based PCR-ribotyping. Sandra Janezic
15:30 Capillary sequencer-based PCR-ribotyping. Alexander Indra
16:00 Coffee break
16:30 Practicals

  • Basic diagnostics (Part 1). Frédéric Barbut & Maja Rupnik
  • DNA extraction and PCR setup for PCR-ribotyping. Sandra Janezic & Alexander Indra
  • In-vitro antimicrobial susceptibility testing. Patrizia Spigaglia

Thursday, 3 September 2015

09:30 Practical: Agarose gel-based PCR-ribotyping. Sandra Janezic
10:30 Coffee break
11:00 Molecular detection of toxin genes. Maja Rupnik
11:30 Practical aspects of in-vitro antimicrobial susceptibility testing.
           Patrizia Spigaglia
12:15 Factors influencing the results of metronidazole resistance testing.
           Elisabeth Nagy
12:45 Lunch break
14:00 Practicals

  • Basic diagnostics (Part 2). Frédéric Barbut & Maja Rupnik
  • PCR-ribotyping – documenting results. Sandra Janezic
  • PCR-ribotyping profile analysis with BioNumerics software. Sandra Janezic
  • PCR-ribotyping profile analysis with WEBRIBO. Alexander Indra
  • Introduction to exercise on comparative genomics. Alexander Indra

18:00 Dinner

Friday, 4 September 2015

09:30 Practicals

  • Basic diagnostics (Part 3). Frédéric Barbut & Maja Rupnik
  • In-vitro antimicrobial susceptibility testing – interpretation of results. Patrizia Spigaglia

11:00 Coffee break
11:30 Solving problems with interpretation of PCR-ribotyping results –                               general discussion. Mark Wilcox
12:30 Lunch break
14:00 Comparative genomics for phylogenetic studies. To be announced
14:45 Comparative genomics for epidemiological investigations. Mark Wilcox
15:30 Practical exercise on comparative genomics. Alexander Indra

Faculty Members
  • Franz Allerberger, Vienna, Austria
  • Frédéric Barbut, Paris, France
  • Alexander Indra, Vienna, Austria
  • Sandra Janezic, Maribor, Slovenia
  • Ed Kuijper, Leiden, Netherlands
  • Elisabeth Nagy, Szeged, Hungary
  • Maja Rupnik, Maribor, Slovenia
  • Patrizia Spigaglia, Rome, Italy
  • Mark Wilcox, Leeds, United Kingdom