2nd Workshop "Antimicrobial Susceptibility Testing and Surveillance: from Laboratory to Clinic - the EUCAST, ESGARS and EPASG Perspective", ESCMID Postgraduate Technical Workshop

25 - 28 September 2012, Madrid, Spain

  • European Committee on Antimicrobial Susceptibility Testing (EUCAST)
  • ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS)
  • ESCMID PK/PD of Anti-Infectives Study Group (EPASG)
Course Coordinators


  • Derek Brown, Peterborough, UK
  • Rafael Cantón, Madrid, ES
  • Gunnar Kahlmeter, Vaxjo, SE


  • Giuseppe Cornaglia, Verona, IT
  • Christian Giske, Stockholm, SE
  • Luis Martínez-Martínez, Santander, ES


  • Alasdair MacGowan, Bristol, UK
  • Johan Mouton, Nijmegen, NL
  • Ursula Theuretzbacher, Vienna, AT
Course Objectives
  • Explain the current process of EUCAST breakpoint definition, understand the rationale for EUCAST breakpoints and explain implementation of EUCAST breakpoints
  • Perform antimicrobial susceptibility testing by dilution, diffusion and automatic techniques, with a EUCAST perspective and explain differences from the CLSI approach
  • Understand the application of EUCAST expert rules for antimicrobial susceptibility testing
  • Explain antimicrobial surveillance systems (public versus private) and benefits for establishment of antimicrobial guidelines and the design of antibiotic policies
  • Understand the importance of breakpoints in antimicrobial resistance surveillance systems
  • Understand how ECOFFs are determined
Course Venue

Microbiology Department
School of Pharmacy
Complutense University
Madrid, Spain

Target Audience

30 – 35 clinical microbiologists with an interest in antimicrobial susceptibility testing and surveillance. Representatives from National Antimicrobial Committees will have preference over other applicants for participation in this PGEC

Contact Persons

Rafael Canton, Madrid, ES

Christian Giske, Stockholm, SE

Luis Martínez-Martínez, Santander, ES

Administrative Secretariat

Ana Moreno-Bofarull
Servicio de Microbiología
Hospital Universitario Ramón y Cajal
Carretera de Colmenar, Km 9,100 

Phone +34 913368832
Fax +34 913368809

Course Programme


Presentations are availabe in the ESCMID Online Lecture Library.



  • Current situation of European (EUCAST) breakpoints. Gunnar Kahlmeter
  • Breakpoints. EUCAST or CLSI? Derek Brown
  • Tools for setting breakpoints: PK/PD and wild type MIC distributions. Johan Mouton
  • Clinical implications of breakpoints. Jesus Rodriguez Baño
  • EUCAST expert rules. Rafael Cantón
  • Antimicrobial susceptibility testing: dilution methods. Luis Martínez-Martínez
  • Antimicrobial susceptibility testing: diffusion methods. Gunnar Kahlmeter
  • Antimicrobial susceptibility testing: automatic systems. Rafael Cantón
  • Haemophilus influenzae – breakpoints and methodology. Erika Matuschek
  • Staphylococcus aureus – susceptibility testing and surveillance of novel antistaphylococcal agents. Robert Skov
  • Detection and characterisation of cephalosporin- and carbapenemresistance in Gram-negative bacilli. Christian Giske
  • Genotypic methods for detection of resistance. Luis Martinez-Martinez
  • Quality assurance of susceptibility testing. Derek Brown
  • Changing from CLSI to EUCAST breakpoints – what happens with surveillance of resistance rates. Maria Isabel Morosini
  • Surveillance of isolates with low-level resistance mechanisms. Alvaro Pascual
  • International surveillance systems – promises and problems. Giuseppe Cornaglia
  • National systems for surveillance of antimicrobial resistance:
    • The Dutch system for surveillance. Nienke van de Sande-Bruinsma
    • The Swedish system for surveillance and early warning. Gunnar Kahlmeter

Practical, Laboratory Sessions

  • International Standards Organisation (ISO) microdilution and other dilution techniques
  • Diffusion techniques (disk diffusion and gradient strips)
  • Automated systems for antimicrobial susceptibility testing
  • Tests for specific resistance mechanisms (ESBLs, AmpC, carbapenemases, hVISA, etc.)
  • Epidemiolgical surveillance computer programmes
  • Computer expert programmes
Course Faculty
  • Derek Brown, Peterborough, UK
  • Rafael Cantón, Madrid, ES
  • Giuseppe Cornaglia, Verona, IT
  • Christian Giske, Stockholm, SE
  • Gunnar Kahlmeter, Vaxjo, SE
  • Luis Martínez-Martínez, Santander, ES
  • Erika Matuschek, Vaxjo, SE
  • Maria-Isabel Morosini, Madrid, ES
  • Johan W. Mouton, Nijmegen, NL
  • Álvaro Pascual, Seville, ES
  • Jesús Rodríguez-Baño, Seville, ES
  • Carlos Ruiz de Alegría, Santander, ES
  • Patricia Ruiz-Garbajosa, Madrid, ES
  • Robert Skov, Copenhagen, DK
  • Nienke van de Sande-Bruinsma, Bilthoven, NL