It is generally recognized that control and prevention of antimicrobial resistance (AMR) requires a holistic One Health approach involving specialists from different sectors. In line with this concept, the 2nd edition of ESCMID ICOHAR will bring together representatives from the public health, medical, veterinary and food safety sectors to share research and education strategies and tools for understanding and reducing the risks of AMR from a One Health perspective. The conference aims at creating an interdisciplinary and intersectorial platform for ongoing dialogue and concerted action among scientists, professionals and policy makers interested in AMR.