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ESCMID Panorama

Medical Biofilm Techniques 2009, ESCMID Postgraduate Technical Workshop

17 - 20 August 2009, Copenhagen, Denmark


Organized by the ESCMID Study Group for Biofilms (ESGB).


  • Niels Hoiby, Copenhagen, Denmark
  • Soren Molin, Copenhagen, Denmark
  • Claus Sternberg, Copenhagen, Denmark
Course Objectives

The course is focused on medical biofilms with examples from urinary tract infections to cystic fibrosis lung infections. The techniques include setup and running of biofilm flow cell systems, fluorescence in situ hybridization, various advanced imaging techniques, image analysis, flow cytometry and demonstration of biofilm techniques in experimental animal research. There will be a morning and a late afternoon lecture, the rest of the day will be laboratory demonstrations and work.


Course Venue

Danish Technical University, Lyngby (Northern part of Copenhagen)


Target Audience

10 - 20 medical doctors with some laboratory training


All presentations are available for ESCMID members. Please log in or register.

Introduction to medical biofilms

  • Notes on the biofilm matrix. Soren Molin
  • Medical biofilms. Niels Hoiby

Introduction to biofilm research

  • Biofilms - the surface-associated worlds. Janus Haagensen
  • Fluorescence in situ hybridization (FISH). Claus Sternberg
  • Peptide nucleic acid fluorescence in situ hybridization. Thomas Bjarnsholt
  • Confocal microscopy: basics and recent innovations. Martin Hoppe

Cystic fibrosis and biofilms

  • Chronic P. aeruginosa biofilm respiratory tract infection in cystic fibrosis - the united airways. Niels Hoiby
  • Clinical and experimental scenarios: immunology. Claus Moser
  • The paranasal sinuses are focus for colonization and chronic biofilm lung infection in cystic fibrosis patients. Helle Krogh Johansen
  • Genetic adaptation by P. aeruginosa to the airways of cystic fibrosis patients. Lars Jelsbak

Urinary tract infections and biofilms. Per Klemm

Quantifying biofilm structure using an image processing programme (COMSTAT). Claus Sternberg

Medical biofilms and antibiotic resistance mechanisms. Niels Hoiby

Animal models of biofilm

  • Introduction. Claus Moser
  • Intra-peritoneal foreign-body infection model in mice. Thomas Bjarnsholt
  • Inflammation during P. aeruginosa lung infection in mice. Peter Ostrup Jensen

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Copenhagen Course Leaflet

Get the Course Leaflet as pdf file