Medical Biofilm Techniques 2012, ESCMID Postgraduate Technical Workshop

27 - 30 August 2012, Copenhagen, Denmark


ESCMID Study Group for Biofilms (ESGB)

Course Objectives

The course is focused on medical biofilms with examples from urinary tract infections to cystic fibrosis lung infections. The techniques include setup and running of biofilm flow cell systems, fluorescence in situ hybridisation, various advanced imaging techniques, image analysis, flow cytometry and demonstration of biofilm techniques in experimental animal research. There will be morning and late afternoon lectures, the rest of the day will include laboratory demonstrations and work.

Course Venue

Danish Technical University
Lyngby, Copenhagen

Target Audience

20 medical doctors with some laboratory training

Administrative Secretariat

Niels Hoiby

Klinisk Mikrobiologisk Afdeling
Rigshospitalet Afsnit 9301
Juliane Maries Vej 22
2100 Copenhagen O

Phone + 45 35 45 77 88
Fax + 45 35 45 64 12

Course Programme


Presentations are availabe in the ESCMID Online Lecture Library.

Day 1

  • Introduction to medical biofilms: antibiotic resistance and general medical importance. Niels Høiby & Søren Molin
  • The exercises and practical and technical information: biofilms and tools, confocal microscopy and COMSTAT, Imaris and FACS. Janus Haagensen & Claus Sternberg
  • Practical: setting up and inoculating biofilm systems

Day 2

  • Cystic fibrosis and biofilms: clinical and experimental scenarios. Niels Høiby, Claus Moser, Helle Krogh Johansen & Lars Jelsbak
  • Medical biofilms and antibiotic resistance mechanisms. Niels Høiby
  • Practical: Confocal microscopy and image acquisition for COMSTAT
  • Practical: Sterilisation of biofilm systems, antibiotic treatment of biofilm systems, washing and reading of microtiter assays, starting up robot microtiter assays

Day 3

  • The role of the matrix in medical biofilms. Søren Molin
  • Demo talk: How to do FISH of biofilms and how to isolate biofilms from flow chambers. Janus Haagensen & Claus Sternberg
  • Practical: Confocal microscopy and image acquisition for COMSTAT
  • Practical: Demonstration of FACS, processing microtiter data, COMSTAT of obtained data, Imaris work on images

Day 4

  • Leica Microsystems: Confocal Microscopy. Martin Hoppe
  • Urinary tract infections and biofilms. Viktoria Hancock
  • General introduction to medical biofilms and animal models of biofilm infections, including Pseudomonas aeruginosa biofilm infections, lung infection models and a peritoneal implant model. Claus Moser, Thomas Bjarnsholt & Peter Østrup Jensen
  • Practical: Evaluation of experiments: mortality/euthanasia, bacteriology, pathology, xenograft model, lung function testing, inflammation/immunology, image processing and demonstration of biofilms from animal models
Course Faculty
  • Thomas Bjarnsholt, Copenhagen, DK
  • Janus Haagensen, Copenhagen, DK
  • Viktoria Hancock, Copenhagen, DK
  • Niels Høiby, Copenhagen, DK
  • Martin Hoppe, Mannheim, DE
  • Lars Jelsbak, Copenhagen, DK
  • Peter Østrup Jensen, Copenhagen, DK
  • Helle Krogh Johansen, Copenhagen, DK
  • Søren Molin, Copenhagen, DK
  • Claus Moser, Copenhagen, DK
  • Claus Sternberg, Copenhagen, DK