2nd Course on Principles of Molecular Microbiological Diagnostics, ESCMID Postgraduate Education Course

20 - 22 January 2016, Maastricht, Netherlands

  • ESCMID Study Group for Molecular Diagnostics (ESGMD)
  • ESCMID Study Group for Epidemiological Markers (ESGEM)
  • Maastricht University Medical Centre

Course Coordinator

Paul Savelkoul, Maastricht, Netherlands

Course Objectives

The course provides an overview of the general principles of state-of-the-art molecular techniques in the diagnosis of infectious diseases. Basic features of e.g. NGS as well as advanced features e.g. real-time PCR will be addressed. In addition, interactive discussions on clinical interpretation and application will be held in several specific simultaneous workshops for which participants will be able to register. Each workshop has a limited number of participants. Registered applicants will have the opportunity to send in their specific questions prior to the course, which will be integrated in the workshop discussions as far as possible. Topics in the same range could be combined. Finally, additional important subjects like laboratory setup, validation and quality control of the different techniques are discussed including future perspectives in the field.

Course Venue

Van der Valk Hotel
Nijverheidsweg 35
6227 AL Maastricht, Netherlands

Target Audience

Up to 100 MDs, (micro)biologists, post-doctoral fellows and PhD students who are working in the field of microbiology and are interested in molecular diagnostics.

Contact Person (Scientific Programme)

Paul Savelkoul
Dept. medical microbiology
P. Debyelaan 25
6229 HX Maastricht

Phone +31 433 876 644
Fax +31 433 876 643

Administrative Secretariat

Fia Claus
Dept. medical microbiology
P. Debyelaan 25
6229 HX Maastricht

Phone +31 433 876 644
Fax +31 433 876 643

Course Programme

Presentations are availabe in the ESCMID Online Lecture Library.

Wednesday, 20 January 2016

09:00 Registration
09:30 Welcome. Paul Savelkoul
10:00 Real-time PCR: the basics. Carl Wittwer
10:30 Quantitation. Jim Huggett
11:00 Coffee break
11:30 Pre-PCR processing. Peter Rådström
12:00 Primer/probe design. Stephen Bustin
12:30 Lunch
13:30 Parallel workshop 1
           PCR: Basics/PCR for starters/case reports. Marijke Raymaekers &
           Marlies Mooij

           Parallel workshop 2
           PCR: Advanced/experienced PCR/case reports. Stephen Bustin &
           Kate Templeton

15:00 Coffee break
15:30 Parallel workshop 3
           Nucleic acid extraction. Eric Claas & Petra Wolffs
           Parallel workshop 4
           Clinical aspects & interpretation of MDx results. Colin Mackenzie &
           Inge van Loo

17:00 Presentations about new developments by companies
18:00 Social programme

Thursday, 21 January 2016

08:30 Highlights of workshops day 1. Lieke van Alphen
09:00 Quality control for molecular diagnostics. Paul Wallace
09:30 Next-generation sequencing. John Rossen
10:00 MALDI-ToF-MS. Hans Kusters
10:30 Coffee break
11:00 Parallel workshop 5
           Basics of NGS. John Rossen & Jacob Moran-Gilad
           Parallel workshop 6
           MALDI-ToF and its clinical applications. Hans Kusters & Eric Claas
12:30 Lunch
13:30 Applications of NGS. Marc Eloit
14:00 Developments in lab automation. Rob Schuurman
14:30 Coffee break
15:00 Parallel workshop 7
           The next step in NGS. Jacob Moran-Gilad & Marc Eloit
           Parallel workshop 8
           Lab automation set-up, creating a lean lab. Rob Schuurman
           & Colin Mackenzie

16:30 Near bed MDx applications. Richard Hodinka
17:00 Molecular detection of antimicrobial resistance. Pierre Bogaerts

Friday, 22 January 2016

08:30 Highlights of workshops day 2. Lieke van Alphen
09:00 Parallel workshop 9
           Broad-range PCR: clinical interpretation of results. Paul Savelkoul
           & Martin Altwegg

           Parallel workshop 10
           CE-IVD, regulations and guidelines. Greet Ieven & Paul Wallace
10:30 Coffee break
11:00 Parallel workshop 11
           Typing, resistance and virulence. Jacob Moran-Gilad & Alexander Mellmann
           Parallel workshop 12
           Molecular diagnostics: when to use (commercial vs. in-house).
           Marijke Raymaekers & Kate Templeton
12:30 Typing in microbiology. Alexander Mellmann
13:00 Future developments in molecular diagnostics. Paul Savelkoul
13:30 Closing lunch
14:30 End of the meeting & departure

Faculty Members
  • Martin Altwegg, Lucerne, Switzerland
  • Pierre Bogaerts, Leuven, Belgium
  • Stephen Bustin, Chelmsford, United Kingdom
  • Eric Claas, Leiden, Netherlands
  • Marc Eloit, Paris, France
  • Richard Hodinka, Greenville, United States
  • Jim Huggett, Teddington, United Kingdom
  • Greet Ieven, Antwerp, Belgium
  • Hans Kusters, Utrecht, Netherlands
  • Colin Mackenzie, Dusseldorf, Germany
  • Alexander Mellmann, Münster, Germany
  • Marlies Mooij, Maastricht, Netherlands
  • Jacob Moran-Gilad, Jerusalem, Israel
  • John Penders, Maastricht, Netherlands
  • Peter Rådström, Lund, Sweden
  • Marijke Raymaekers, Hasselt, Belgium
  • John Rossen, Groningen, Netherlands
  • Paul Savelkoul, Maastricht, Netherlands
  • Rob Schuurman, Utrecht, Netherlands
  • Kate Templeton, Edinburgh, United Kingdom
  • Lieke van Alphen, Maastricht, Netherlands
  • Inge van Loo, Maastricht, Netherlands
  • Paul Wallace, Glasgow, United Kingdom
  • Carl Wittwer, Salt Lake City, UT, United States
  • Petra Wolffs, Maastricht, Netherlands