CMI highlight: Effective chemical virus inactivation of patient serum compatible with accurate serodiagnosis of infections
Remy et al aimed to assess the accuracy of 19 serological tests after chemical (Triton X-100 1%, 0.1%, and 0.1%) or thermal (60°C, 60 minutes) inactivation of patient serum samples containing herpes simplex virus-1 (HSV-1).
Serological measurements showed good agreement between controls and samples treated with Triton X-100 0.1% and 1%, with an estimated bias of -0.6±9.2% (n=258) and -0.1±18.6% (n=174), respectively. Discordant qualitative results were rare. Conversely, heat inactivation alone, and combined with Triton X-100 0.1% triggered a bias of 17.5±66.4% (n=200) and 37.9±79.8% (n=160), respectively. Triton X-100 1% completely inactivated HSV-1 in 1% and 90% serum while Triton X-100 0.1% failed to do so in 90% serum.
In conclusion, the results indicate that unlike heat inactivation, Triton X-100 1% enabled accurate serological testing and completely inactivated HSV-1 in serum, allowing for a simple and safe method to routinely diagnose high-risk patients. Read more